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</ul></div> <div id="sssawse2" class="sublanmu_box sublanmu_box_806" id="sublanmu_box"> <div id="sssawse2" class="sublanmu_content" id="sublanmu_content"> <div id="sssawse2" class="articleinfor" id="articleinfor"> <ul> <li id="sssawse2" class="title"><h1>选择液相色谱保护柱的建议 </h1></li> <li id="sssawse2" class="info"> 发布日期：2020-07-07 作者：科学仪器信息网浏览量：<span id="clicks"></span> 字号：[ <a href="javascript:FontZoom('16px','Content')">大</a> <a href="javascript:FontZoom('14px','Content')">中</a> <a href="javascript:FontZoom('12px','Content')">小</a>] </li> </ul> <div id="sssawse2" class="content" id="Content"> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">在高效液相分析检测样品的过程中，色谱柱会受到来自于样品及色谱系统的污染，从而导致色谱柱耐用性差、寿命缩短。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">来自于色谱系统的污染主要指，HPLC仪器系统中部件磨损而产生的固体颗粒，以及流动相系统过滤不完全残留的固体颗粒。来自于样品的污染主要指，未完全溶解的样品或者已完全溶解的样品进入色谱系统中，由于样品溶剂和流动相溶剂对样品溶解存在的溶解度差异，导致沉淀析出污染系统。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">特别是对于中药、天然产物、合成中间体及生物药品的检测，因基质的复杂性，色谱柱受污染的情况会更加严重。污染会导致色谱系统压力升高、色谱柱塌陷、填料变色等，从而带来检测成本高，测试结果不准确等各种影响。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">图：系统压力升高或样品基质复杂导致分析柱污染</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <img style="font-size:16px;max-width:100%;border-top:0px;height:auto;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:10px auto 0px;display:block;padding-right:0px;" src="/e/upload/s1/fck/image/2020/07/c-1ec8b3a75e2843a3a83bd3a794c43d1d_th.png" /> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;"><a href="http://www.bugsrc.cn/index.aspx?lanmuid=89&sublanmuid=777" target="_blank">?；ぶ?/a>是液相色谱分析中重要的色谱耗材，使用?；ぶ纱蟠蠼档头治鲋芪廴镜某潭?，延长分析柱使用寿命。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">?；ぶ挥诮饔敕治鲋?，作用主要两方面：</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">1. 截留不溶性颗粒</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">2. 吸附样品基质中的杂质</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">以下几种情况建议使用?；ぶ?/span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">1. 中药、天然产物及合成中间体等基质较复杂的样品分析——因溶解性差异导致样品析出而污染分析柱的情况比较严重，梯度洗脱时影响更大。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">2. 生物样品分析——因样品的不可逆吸附导致的污染，?；ぶ上扔诜治鲋惺苷庵治廴?，从而延长分析柱寿命。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">?；ぶ苤匾≡窈鲜实谋；ぶ匾?，选择或使用不当也可能带来截然相反的效果。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">?；ぶ掷嗉把≡瘢?/span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">从材质上分：不锈钢材质和PEEK（聚醚醚酮）材质</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <img style="font-size:16px;max-width:100%;border-top:0px;height:auto;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:10px auto 0px;display:block;padding-right:0px;" src="/e/upload/s1/fck/image/2020/07/c-275d6bab492b415c85938a93ccbb569a.jpg" /> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">PEEK 材质优缺点——样品对金属离子比较敏感时，选择PEEK材质比较合适，比如某些蛋白分析会用到PEEK材质的分析柱，主要就是考虑到这方面的因素；</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">不锈钢材质优缺点——大部分小分子化合物的分析，选择不锈钢材质?；ぶ佣?，特别是当流动相体系使用到四氢呋喃（THF）或DMSO等一些溶解性较强的溶剂时，必须选择不锈钢材质，甚至对于HPLC系统的管路也需要替换成不锈钢材质，因THF对于PEEK管路有相当强的腐蚀作用。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">从结构上分：卡套式（柱芯+柱套）和直接填装短柱</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">直接填装短柱优缺点——这种结构的?；ぶ抵示褪嵌痰纳字?，一般填装的填料跟分析柱填料完全一样。生物样品分析中，用这种类型保护柱居多，除可过滤一些不溶颗粒外，还可除去一些在分析柱上易吸附的杂质。缺点是，一次性使用，污染严重时，只能报废。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">卡套式保护柱优缺点——卡套式?；ぶ闹卓煞锤词褂?，柱芯污染后可替换，厂家会提供配套的保护柱芯，但购买时需要注意，柱芯中有与分析柱匹配的填料，需要确认柱芯中的填料类型再购买。同时，柱芯和柱套需是来源于同一厂家的同一规格，否则，会存在匹配上的差异，导致峰型异?；蛘呗┮?。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">从连接方式上分：独立式和直连式</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <img style="font-size:16px;max-width:100%;border-top:0px;height:auto;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:10px auto 0px;display:block;padding-right:0px;" src="/e/upload/s1/fck/image/2020/07/c-54ccafbc4235466db34965faf76a490e.jpg" /> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">独立式保护柱优缺点</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">——?；ぶ头治鲋溆靡欢喂苈妨樱挥玫Ｐ挠敕治鲋诹由系钠ヅ湮侍?。但使用管路连接时，连接不当容易产生死体积。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">直连式?；ぶ湃钡恪；ぶ涌诖菸?，可直接与分析柱连接，死体积小，UPLC分析时建议选择直连式?；ぶ蝗钡悖汗郝蚴毙璞Ｖそ涌谟敕治鲋ヅ?。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">保护柱选择考虑以下几方面因素：</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">1. 除非特殊情况，一般选择填料基质与分析柱基质完全相同的保护柱。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">2. 选择?；ぶ叽缈砂粗寤扑?，?；ぶ寤捶治鲋寤?%~10%即可；如4.6*250mm的分析柱体积约为4mL，?；ぶ寤裳≡裨?.2~0.4mL之间为宜。同时，?；ぶ诰队τ敕治鲋诰断嗤蛳嗟?。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">?；ぶ褂米⒁馐孪睿?/span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">1. 独立式?；ぶ敕治鲋邮?，连接管路尽可能短，且接口切面平滑，尽量减少死体积以降低柱外展宽效应。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">2. 卡套式?；ぶ话阄幢曜⒁毫鞣较颍褂檬弊詈米孕斜曜?，并按照标注方向来使用，以免正反向使用后，截留的杂质污染分析柱。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">3. 直接填装的短柱型保护柱，出现柱压升高或峰型变差时，可不接分析柱进行反向清洗，清洗溶剂可参考分析柱清洗溶剂。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">4. 卡套式?；ぶ咎婊皇?，注意柱芯与柱套是否存在方向上的匹配，同时不易旋拧过紧，以防滑丝。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">5. ?；ぶ蛑疽话悴唤ㄒ槌逑?，因可能对填料表面修饰有影响。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">6. 如同一?；ぶ洳煌姆治鲋褂?，每次使用前，?；ぶ柘惹逑床⒂昧鞫嗥胶獗；ぶ欢问奔浜?，再接分析柱使用。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">?；ぶ胫肮似鞯那鸷褪褂茫?/span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">使用保护柱的目的在于延长分析柱的使用寿命，当分析柱造价昂贵时，?；ぶ募壑涤绕淠芴逑?，某些情况下，当使用的分析柱本身价格便宜，保护柱并非必选，推荐使用柱前过滤器更合适。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">柱前过滤器与?；ぶ秸咄庑蜗嗨?，差异主要在于：?；ぶ谛咀坝刑盍希肮似髂谛臼强筛坏穆似ㄒ话阄椎牟恍飧稚战岚澹?/span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">因此，从结构可以推测使用上的差异：</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">柱前过滤器——通用性强，任何分析柱前都可使用，因其滤芯只起到过滤和截留杂质的作用，滤片污染后可超声清洗，反复使用。</span> </p> <p style="font-size:16px;border-top:0px;font-family:"border-right:0px;white-space:normal;word-spacing:0px;border-bottom:0px;text-transform:none;font-weight:400;color:#191919;padding-bottom:0px;font-style:normal;text-align:left;padding-top:0px;padding-left:0px;border-left:0px;orphans:2;widows:2;margin:0.63em 0px 1.8em;letter-spacing:normal;counter-reset:list-1 0 list-2 0 list-3 0 list-4 0 list-5 0 list-6 0 list-7 0 list-8 0 list-9 0;padding-right:0px;background-color:#ffffff;text-indent:0px;font-variant-ligatures:normal;font-variant-caps:normal;-webkit-text-stroke-width:0px;text-decoration-style:initial;text-decoration-color:initial;"> <span style="font-size:16px;border-top:0px;border-right:0px;border-bottom:0px;padding-bottom:0px;padding-top:0px;padding-left:0px;border-left:0px;margin:0px;padding-right:0px;">保护柱——柱中填料除了起截留杂质的作用外，也可除去样品中易吸附的杂质，这一点对生物样品分析尤其重要。</span> </p> </div> </div> <script src="/e/js/internal_page.js" type="text/javascript"></script> <ul class="sublanmu_pn"> <li>上一篇：<a href="/index.aspx?lanmuid=63&sublanmuid=806&id=1827">色谱柱超级保养心得</a></li> <li>下一篇：<a href="/index.aspx?lanmuid=63&sublanmuid=806&id=1842">又见婴儿毒奶粉？</a></li> </ul> <script type="text/javascript">Get_Info("article","1828")</script> </div></div></div> </div> <div id="sssawse2" class="clear main_box_inner_bottom"></div> </div> <div id="sssawse2" class="bottom_box"><dl class="db_ba"> <dd class="db_left"> <span 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